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| Kavalco,Karine Frehner; Brandão,Karina de Oliveira; Pazza,Rubens; Almeida-Toledo,Lurdes Foresti de. |
| Four populations of Astyanax hastatus Myers 1928 from the Guapimirim River basin (Rio de Janeiro State) were analyzed and three distinct cytotypes identified. These cytotypes presented 2n = 50 chromosomes, with 4M+8SM+10ST+28A (Cytotype A), 8M+10SM+14ST+18A (Cytotype B), 6M+8SM+4ST+32A (Cytotype C) and scanty heterochromatin, mainly located throughout pericentromeric regions of several chromosomal pairs. No homologies with the As-51 satellite DNA were observed in the three cytotypes, although all of them presented multiple 18S rDNA sites, as detected by both silver nitrate staining and FISH (fluorescent in situ hybridization). The application of the term "species complex" in Astyanax is discussed from a cytotaxonomic viewpoint. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Astyanax hastatus; Molecular cytogenetics; Karyotypic evolution; Species complex. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300009 |
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| Bülau,Sandra Eloisa; Kretschmer,Rafael; Gunski,Ricardo José; Garnero,Analía del Valle; O’Brien,Patricia C. M.; Ferguson-Smith,Malcolm A.; Oliveira,Edivaldo Herculano Correa de; Freitas,Thales Renato Ochotorena de. |
| Abstract Zonotrichia capensis is widely distributed in the Neotropics. Previous cytogenetic studies demonstrated the presence of polymorphisms in two chromosome pairs (ZCA2 and ZCA4). Here, we report results based on comparative chromosome painting, using probes derived from Gallus gallus and Leucopternis albicollis, focused on characterizing the chromosome organization of Z. capensis. Our results demonstrate the conservation of ancestral syntenies as observed previously in other species of passerine. Syntenies were rearranged by a series of inversions in the second chromosome as described in other Passeriformes, but in this species, by using probes derived from L. albicollis we observed an extra inversion in the second chromosome that had not previously... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Birds; Chromosomal rearrangements; Molecular cytogenetics; FISH. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500799 |
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| Barcellos,Suziane Alves; Kretschmer,Rafael; Souza,Marcelo Santos de; Costa,Alice Lemos; Degrandi,Tiago Marafiga; Lopes,Cassiane Furlan; Ferguson-Smith,Malcolm A.; Pereira,Jorge; Oliveira,Edivaldo Herculano Correa de; Gunski,Ricardo José; Garnero,Analía del Valle. |
| Abstract Despite the richness of species in the Hirudinidae family, little is known about the genome organization of swallows. The Progne tapera species presents genetic and morphological difference when compared to other members of the same genus. Hence, the aims of this study were to analyze the chromosomal evolution of three species Progne tapera, Progne chalybea and Pygochelidon cyanoleuca - by comparative chromosome painting using two sets of probes, Gallus gallus and Zenaida auriculata, in order to determine chromosome homologies and the relationship between these species. All karyotypes exhibited 76 chromosomes with similar morphology, except for the 5th, 6th and 7th chromosome pairs in P. cyanoleuca. Additionally, comparative chromosome painting... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Homology; Molecular cytogenetics; Fluorescent in situ hybridization; Hirundinidae. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100201 |
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| Oliveira,Érica Cristina de; Pinto-Maglio,Cecília Alzira Ferreira. |
| ABSTRACT: Presence of the ALMT1 (aluminum-activated malate transporter) gene confers resistance to aluminum toxicity in Triticum aestivum (common wheat). No resistant cultivars of Triticum turgidum ssp. Durum Desf. (durum wheat) have been registered in Brazil. The aim of this study was to map the ALMT1 through application of the FISH (fluorescence in situ hybridization) technique in five wheat genotypes, common and durum, from the Active Germplasm Bank (AGB) of the Instituto Agronômico (IAC): BH 1146, P19, P33, Anahuac, and IAC 1003. FISH-ALMT1 signals were registered in Anahuac (sensitive) chromosomes and in BH 1146, P19, and P33 (resistant) chromosomes. In the three resistant genotypes, a characteristic double FISH signal was found, located in different... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Triticum sp.; Molecular cytogenetics; Fluorescence in situ hybridization; Aluminum tolerance; Toxicity. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162020000501102 |
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| Bellini,Marilanda Ferreira; Silva,Ana Elizabete; Varella-Garcia,Marileila. |
| This review summarizes the chromosomal changes detected by molecular cytogenetic approaches in esophageal squamous cell carcinoma (ESCC), the ninth most common malignancy in the world. Whole genome analyses of ESCC cell lines and tumors indicated that the most frequent genomic gains occurred at 1, 2q, 3q, 5p, 6p, 7, 8q, 9q, 11q, 12p, 14q, 15q, 16, 17, 18p, 19q, 20q, 22q and X, with focal amplifications at 1q32, 2p16-22, 3q25-28, 5p13-15.3, 7p12-22, 7q21-22, 8q23-24.2, 9q34, 10q21, 11p11.2, 11q13, 13q32, 14q13-14, 14q21, 14q31-32, 15q22-26, 17p11.2, 18p11.2-11.3 and 20p11.2. Recurrent losses involved 3p, 4, 5q, 6q, 7q, 8p, 9, 10p, 12p, 13, 14p, 15p, 18, 19p, 20, 22, Xp and Y. Gains at 5p and 7q, and deletions at 4p, 9p, and 11q were significant prognostic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: CGH; Esophageal carcinoma; FISH; Genomic imbalances; Molecular cytogenetics. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200001 |
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| Riegel,Mariluce. |
| The field of cytogenetics has focused on studying the number, structure, function and origin of chromosomal abnormalities and the evolution of chromosomes. The development of fluorescent molecules that either directly or via an intermediate molecule bind to DNA has led to the development of fluorescent in situ hybridization (FISH), a technology linking cytogenetics to molecular genetics. This technique has a wide range of applications that increased the dimension of chromosome analysis. The field of cytogenetics is particularly important for medical diagnostics and research as well as for gene ordering and mapping. Furthermore, the increased application of molecular biology techniques, such as array-based technologies, has led to improved resolution,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Molecular cytogenetics; FISH; Array-CGH; Copy number variation; Genomic disorders. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000200006 |
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| REIS,ARYANE C.; FRANCO,ANA LUIZA; CAMPOS,VICTÓRIA R.; SOUZA,FLÁVIA R.; ZORZATTO,CRISTIANE; VICCINI,LYDERSON F.; SOUSA,SAULO M.. |
| ABSTRACT Agapanthus (Agapanthaceae) has 10 species described. However, most taxonomists differ respect to this number because the great phenotypic plasticity of the species. The cytogenetic has been an important tool to aid the plant taxon identification, and to date, all taxa of Agapanthus L'Héritier studied cytologically, presented 2n = 30. Although the species possess large chromosomes, the group is karyologically little explored. This work aimed to increase the cytogenetic knowledge of Agapanthus africanus (L.) Hoffmanns by utilization of chromosome banding techniques with DAPI / CMA3 and Fluorescent in situ Hybridization (FISH). In addition, flow cytometry was used for determination of DNA content and the percentage of AT / GC nitrogenous bases.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: African lily; Chromosome banding; Cytotaxonomy; Flow cytometry; Genome size; Molecular cytogenetics. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000501727 |
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| Ventura,Karen; Moreira,Camila N; Moretti,Renata; Yonenag-Yassuda,Yatiyo; Rodrigues,Miguel T. |
| The karyotype of the big-headed Amazon River turtle, Peltocephalus dumerilianus, is characterized based on a sample of seven juveniles from Reserva Biológica do Rio Trombetas, Pará State, Brazil (1°30' S, 56°34' W). Here we present the first results on GTG and CBG-banding patterns, Ag-NOR staining and FISH, with telomeric and 45S rDNA sequences as probes. A cytogenetic comparison with related Podocnemidae is also provided. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Big-headed Amazon River turtle; Molecular cytogenetics; Pelomedusoides. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100011 |
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